期刊
ACS NANO
卷 4, 期 11, 页码 6587-6598出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn1018279
关键词
biocompatibility; carbon nanotubes; cell adhesion; gene transfection; graphene
类别
资金
- Korean government (MEST) [313 2008 2 C00538 2008 0062074]
- MEST [2004457]
- Ministry of Education Science and Technology in Korea
- National Research Foundation of Korea [313-2008-2-C00538, 2008-0062074] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Carbon based materials, including graphene and carbon nanotubes, have been considered attractive candidates for biomedical applications such as scaffolds in tissue engineering, substrates for stem cell differentiation, and components of implant devices. Despite the potential biomedical applications of these materials, only limited information is available regarding the cellular events, including cell viability, adhesion, and spreading, that occur when mammalian cells interface with carbon based nanomaterials. Here, we report behaviors of mammalian cells, specifically NIH 3T3 fibroblast cells, grown on supported thin films of graphene and carbon nanotubes to investigate biocompatibility of the artificial surface Proliferation assay cell shape analysis, focal adhesion study and quantiative measurements of cell adhesion related gene expression levels by RT PCR reveal that the fibroblast cells grow well, with different numbers and sizes of focal adhesions, on graphene- and carbon nanotube coated substrates Interestingly, the gene transfection efficiency of cells grown on the substrates was improved up to 250% that of cells groin on a cover glass. The present study suggests that these nanomaterials hold high potential for bioapplications showing high biocompatibility, especially as surface coating materials for implants, without inducing notable deleterious effects while enhancing some cellular functions (i e, gene transfection and expression)
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