Functional Assessment of Metal Oxide Nanoparticle Toxicity in Immune Cells

Understanding the nanoparticle cell interaction is critical for the safe development of nanomaterials. Herein, we explore the impact of three metal oxide nanoparticles, nonporous Stober SiO2, mesoporous SiO2, and nonporous anatase TiO2 nanoparticles, on primary culture mast cells. Using transmission electron microscopy and inductively coupled plasma atomic emission spectroscopy, we demonstrate that each class of nanoparticle is internalized by the mast cells, localizing primarily in the secretory granules, with uptake efficiency increasing in the following order: nonporous SiO2 < porous SiO2 < nonporous TiO2 nanoparticles. The influence of nanoparticle-laden granules was assessed using carbon-fiber microelectrode amperometry measurements that reveal functional changes in chemical messenger secretion from mast cell granules. Both nonporous and porous SiO2 nanoparticles cause a decrease in the number of molecules released per granule, with nonporous SiO2 also inducing a decrease in the amperometric spike frequency and, therefore, having a larger impact on cell function. As the two classes of SiO2 nanoparticles vary only in their porosity, these results suggest that, while the mesoporous SiO2 has a drastically larger total surface area due to the pores, the cell-contactable surface area, which is higher for the nonporous SiO2, is more important in determining a nanoparticles' cellular impact. In comparison, exposure to nonporous TiO2 slows the kinetics of secretion without altering the number of molecules released from the average granule. The varying immune cell response following exposure to nonporous SiO2 and nonporous TiO2 indicates that the nanoparticle cell interactions are also modulated by surface chemistry.