期刊
ACS NANO
卷 4, 期 3, 页码 1511-1520出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn9017334
关键词
self-assembly; gold; nanoparticles; near-infrared; fluorescence; imaging; dark quencher; proteolysis; cancer; tumor
类别
资金
- Whitaker Foundation
- NIH [CA119402, DE013023, DE016516]
- National Science Foundation [DMR-08-19762]
Target-activatable fluorogenic probes based on gold nanoparticles (AuNPs) functionalized with self-assembled heterogeneous monolayers of dye-labeled peptides and poly(ethylene glycol) have been developed to visualize proteolytic activity in vivo. A one-step synthesis strategy that allows simple generation of surface-defined AuNP probe libraries is presented as a means of tailoring and evaluating probe characteristics for maximal fluorescence enhancement after protease activation. Optimal AuNP probes targeted to trypsin and urokinase-type plasminogen activator required the incorporation of a dark quencher to achieve 5- to 8-fold signal amplification. These probes exhibited extended circulation time in vivo and high image contrast in a mouse tumor model.
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