期刊
ACS NANO
卷 3, 期 8, 页码 2163-2170出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn900187k
关键词
nanobiotechnology; bionanotechnology; viral DNA packaging; phi29 DNA packaging motor; protein nanoparticles; virus assemble; bacteriophage phi29 connector
类别
资金
- NIH [EB003730, GM59944, EY018230, RR022200]
- NSF [TG-MCB070038]
A 24 x 30 nm ellipsoid nanoparticle containing 84 subunits or 7 dodecamers of the re-engineered core protein of the bacteriophage phi29 DNA packaging motor was constructed. Homogeneous nanoparticles were obtained with simple one-step purification. Electron microscopy and analytical ultracentrifugation were employed to elucidate the structure, shape, size, and mechanism of assembly. The formation of this structure was mediated and stabilized by N-terminal peptide extensions. Reversal of the 84-subunit ellipsoid nanoparticle to its dodecamer subunit was controlled by the cleavage of the extended N-terminal peptide with a protease. The 84 outward-oriented C-termini were conjugated with a streptavidin binding peptide which can be used for the incorporation of markers. This further extends the application of this nanoparticle to pathogen detection and disease diagnosis by signal enhancement.
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