期刊
ACS NANO
卷 2, 期 11, 页码 2401-2409出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn800592q
关键词
microparticles; drug loading; controlled drug release; cancer therapy; fluorescence resonant energy transfer; nanotechnology
类别
资金
- National Cancer Institute of the National Institutes of Health (NIH) [U54 CA 119335]
- National Science Foundation [DMR-0806859]
- CNRS/DREI
- [2005 3312]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [0806859] Funding Source: National Science Foundation
The fluorescent dye Alexa Fluor 488 or the anticancer drug doxorubicin is attached to the surface and inner pore walls of mesoporous Si particles by covalent attachment, and the oxidation-induced release of each molecule is studied. The molecules are bound to the Si matrix using a 10-undecenoic acid linker, which is attached by thermal hydrosilylation. Loading capacity of the microparticles using this method is similar to 0.5 and 45 mg/g of porous Si microparticle for Alexa Fluor 488 and doxcrubicin, respectively. The Si -(-bound assembly is initially stable in aqueous solution, although oxidation of the underlying Si matrix results in conversion to silicon oxide and slow release of the linker-molecule complex by hydrolysis of the Si-O attachment points. When the attached molecule is a fluorophore (Alexa Fluor 488 or doxorubicin), its fluorescence is effectively quenched by the semiconducting silicon matrix. As the particle oxidizes in water, the fluorescence intensity of the attached dye increases due to growth of the insulating silicon oxide layer and, ultimately, dye release from the surface. The recovery of fluorescence in the microparticle and the release of the molecule into solution are monitored in real-time by fluorescence microscopy. Both processes are accelerated by introduction of the oxidizing species peroxynitrite to the aqueous solution. The oxidation-triggered release of the anticancer drug doxorubicin to HeLa cells is demonstrated.
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