期刊
ACS CHEMICAL NEUROSCIENCE
卷 10, 期 1, 页码 304-312出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschemneuro.8b00248
关键词
Nanoparticle; silica; reactive oxygen species; cytotoxicity; neuron; stress
资金
- KAKENHI [26460707, 17K09040, 26670127, 16H04667]
- Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT)
- MEXT
- Foundation of Strategic Research Projects in Private Universities from MEXT [S1311011]
- Institute for Environmental & Gender-specific Medicine Juntendo University
- Nakatomi Foundation
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [16H04667, 17K09040, 26460707, 26670127] Funding Source: KAKEN
Silica nanoparticles (SiNPs) are produced on an industrial scale and used in various fields including health care, because silica is stable, inexpensive, and easy to handle. Despite these benefits, there is concern that exposure to SiNPs may lead to adverse effects in certain types of cells or tissues, such as hemolysis, immune responses, and developmental abnormalities in the brain and developing embryos. Although investigations on the toxicity of SiNPs against neurons are essential for medicinal use, only a few studies have assessed the direct effects of SiNPs on cells derived from the central nervous system. In this study, we investigated the toxic effects of SiNPs on primary cultures of hippocampal cells, using SiNPs with diameters of 10-1500 nm. We showed that treatment with SiNPs caused oxidative stress and cell death. Furthermore, we found that these cytotoxicities were dependent on the particle size, concentration, and surface charge of SiNPs, as well as the treatment temperature. The toxicity was reduced by SiNP surface functionalization or protein coating and by pretreating cells with an antioxidant, suggesting that contact-induced oxidative stress may be partially responsible for SiNP-induced cell death. These data will be valuable for utilizing SiNPs in biomedical applications.
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