期刊
ACS CHEMICAL NEUROSCIENCE
卷 1, 期 1, 页码 13-18出版社
AMER CHEMICAL SOC
DOI: 10.1021/cn900015v
关键词
Amyloid; aggregation; kinetics; mechanism; Alzheimer; fibril
资金
- Swedish Research Council
- Greta och Johan Kock's Foundation
- Wellcome Trust [067660]
Protein aggregation can lead to major disturbances of cellular processes and is associated with several diseases. We report kinetic and equilibrium data by ThT fluorescence and enzyme-linked immunosorbent assay of sufficient quality and reproducibility to form a basis for mechanistic understanding of amyloid beta-peptide (A beta) fibril formation. Starting from monomeric peptide in a pure buffer system without cosolvents, we find that the kinetics of A beta aggregation vary strongly with peptide concentration in a highly predictable manner. The free A beta concentration in equilibrium with fibrils was found to vary with total peptide concentration in a manner expected for a two-phase system. The free versus total A beta concentration was linear up to ca. 0.2,mu M, after which free A beta decreased with total A beta toward an asymptotic value. Our results imply that A beta fibril formation arises from a sequence of events in a highly predictable manner.
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