期刊
ACS CHEMICAL BIOLOGY
卷 9, 期 9, 页码 2014-2022出版社
AMER CHEMICAL SOC
DOI: 10.1021/cb500324n
关键词
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资金
- NIH [DP2 OD008463]
- David and Lucile Packard Fellowship
- Robert C. and Carolyn J. Springborn Endowment
- American Society for Biochemistry and Molecular Biology Undergraduate Research Award
- Institute for Genomic Biology
- National Center for Research Resources, National Institutes of Health [S10 RR027109 A]
Natural products remain an important source of drug candidates, but the difficulties inherent to traditional isolation, coupled with unacceptably high rates of compound rediscovery, limit the pace of natural product detection. Here we describe a reactivity-based screening method to rapidly identify exported bacterial metabolites that contain dehydrated amino acids (i.e., carbonyl- or imine-activated alkenes), a common motif in several classes of natural products. Our strategy entails the use of a commercially available thiol, dithiothreitol, for the covalent labeling of activated alkenes by nudeophilic 1,4-addition. Modification is easily discerned by comparing mass spectra of reacted and unreacted cell surface extracts. When combined with bioinformatic analysis of putative natural product gene clusters, targeted screening and isolation can be performed on a prioritized list of strains. Moreover, known compounds are easily dereplicated, effectively eliminating superfluous isolation and characterization. As a proof of principle, this labeling method was used to identify known natural products belonging to the thiopeptide, lanthipeptide, and linaridin classes. Further, upon screening a panel of only 23 actinomycetes, we discovered and characterized a novel thiopeptide antibiotic, cydothiazomycin C.
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