期刊
ACS CHEMICAL BIOLOGY
卷 9, 期 4, 页码 884-890出版社
AMER CHEMICAL SOC
DOI: 10.1021/cb400917a
关键词
-
资金
- National Institute of Health [1R01CA161158]
- National Science Foundation [CHEM-1148684]
- Welch Foundation [A-1715]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1148684] Funding Source: National Science Foundation
Seven phenylalanine derivatives with small ortho substitutions were genetically encoded in Escherichia coli and mammalian cells at an amber codon using a previously reported, rationally designed pyrrolysyl-tRNA synthetase mutant (PylRS-(N346A/C348A)) coupled with tRNA(CUA)(Pyl). Ortho substitutions of the phenylalanine derivatives reported herein include three halides, methyl, methoxy, nitro, and nitrile. These compounds have the potential for use in multiple biochemical and biophysical applications. Specifically, we demonstrated that o-cyano-phenylalanine could be used as a selective sensor to probe the local environment of proteins and applied this to study protein folding/unfolding. For six of these compounds this constitutes the first report of their genetic incorporation in living cells. With these compounds the total number of substrates available for PylRS(N346A/C348A) is increased to nearly 40, which demonstrates that PylRS(N346A/C348A) is able to recognize phenylalanine with a substitution at any side-chain aromatic position as a substrate. To our knowledge, PylRS(N346A/C348A) is the only aminoacyl-tRNA synthetase with such a high substrate promiscuity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据