期刊
ACS CHEMICAL BIOLOGY
卷 9, 期 4, 页码 867-873出版社
AMER CHEMICAL SOC
DOI: 10.1021/cb400925k
关键词
-
资金
- Max Planck Society
- Korber Foundation
- German Federal Ministry of Education and Research [0315447]
- German Research Foundation through an Emmy Noether fellowship [RA1944/2-1]
- Fonds der Chemischen Industrie
- Studienstiftung des Deutschen Volkes
Today, the process of selecting carbohydrate antigens as a basis for active vaccination and the generation of antibodies for therapeutic and diagnostic purposes is based on intuition combined with trial and error experiments. In efforts to establish a rational process for glycan epitope selection, we employed glycan array screening, surface plasmon resonance, and saturation transfer difference (STD)-NMR to elucidate the interactions between antibodies and glycans representing the Yersinia pestis lipopolysaccharide (LPS). A trisaccharide epitope of the LPS inner core glycan and different LPS-derived oligosaccharides from various Gram-negative bacteria were analyzed using this combination of techniques. The antibody-glycan interaction with a heptose substructure was determined at atomic-level detail. Antibodies specifically recognize the Y. pestis trisaccharide and some substructures with high affinity and specificity. No significant binding to LPS glycans from other bacteria was observed, which suggests that the epitopes for just one particular bacterial species can be identified. On the basis of these results we are beginning to understand the rules for structure-based design and selection of carbohydrate antigens.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据