期刊
ACS CHEMICAL BIOLOGY
卷 7, 期 11, 页码 1807-1816出版社
AMER CHEMICAL SOC
DOI: 10.1021/cb300342u
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资金
- U.K. Medical Research Council [U1051922716]
- U.S. National Science Foundation [MCB 0722787]
- National Institutes of Health [GM070641]
- MRC [MC_U105192716] Funding Source: UKRI
- Medical Research Council [MC_U105192716] Funding Source: researchfish
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1120346] Funding Source: National Science Foundation
C-1 carriers are essential cofactors in all domains of life, and in Archaea, these can be derivatives of tetrahydromethanopterin (H-4-MPT) or tetrahydrofolate (H-4-folate). Their synthesis requires 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP) as the precursor, but the nature of pathways that lead to its formation were unknown until the recent discovery of the GTP cyclohydrolase IB/MptA family that catalyzes: the first: step, the conversion of GTP to dihydroneopterin 2',3'-cyclic phosphate or 7,8-dihydroneopterin triphosphate [El Yacoubi, B.; et aL (2006) J. Biol 281, 37586-37593 and Grochowski, L.-L.; et al. (2007) Biochemistry 46, 6658-6667]. Using a combination of comparative genomics analyses, heterologous complementation tests, and in vitro assays;,we-show that the archaeal protein families COG2098 and COG1634 specify two of the missing 6-HMDP synthesis enzymes. Members of the COG2098 family catalyze the formation of 6-hydroxymethyl-7,8-dihydropterin from 7,8-dihydroneopterin, while members of the COG 1634 family catalyze the formation of 6-HMDP from 6-hydroxymethyl-7,8-dihydropterin. The discovery of these missing genes solves a long-standing mystery and provides novel example's of convergent evolutions Where Proteins of dissimilar architectures perform the same biochemical function.
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