期刊
ACS CHEMICAL BIOLOGY
卷 6, 期 1, 页码 34-46出版社
AMER CHEMICAL SOC
DOI: 10.1021/cb100294v
关键词
2D-PAGE; Affinity chromatography; Degradomics; Drug affinity responsive target stability (DARTS); MudPIT; SILAC; Target identification
资金
- National Institutes of Health [R01 CA124974, R21 CA149774, R01 AA007680]
- American Cancer Society [RSG-07-035-01-CCG]
- NATIONAL CANCER INSTITUTE [R01CA124974, R21CA149774] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM008496] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM [R01AA007680] Funding Source: NIH RePORTER
Small-molecule target identification is a vital and daunting task for the chemical biology community as well as for researchers interested in applying the power of chemical genetics to impact biology and medicine. To overcome this target ID bottleneck, new technologies are being developed that analyze protein drug interactions, such as drug affinity responsive target stability (DARTS), which alms to discover the direct binding targets (and off targets) of small molecules on a proteome scale without requiring chemical modification of the compound. Here, we review the DARTS method, discuss why it works, and provide new perspectives for future development in this area.
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