期刊
ACS CHEMICAL BIOLOGY
卷 6, 期 6, 页码 563-572出版社
AMER CHEMICAL SOC
DOI: 10.1021/cb100392r
关键词
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资金
- American Lung Association
- Public Health Services [T32 CA09302]
- U.S. National Cancer Institute, Department of Health and Human Services
- National Institutes of Health [R01 EB005011]
- American Asthma Foundation
Cathepsin X is a lysosomal cysteine protease that functions as a carboxypeptidase with broad substrate specificity. Cathepsin X was discovered only recently, and its physiological roles are still not well understood. A number of studies suggest that cathepsin X may be involved in a variety of biological processes, including cancer, aging and degenerative conditions of the brain, inflammation, and cellular communication. Here we present the synthesis and characterization of several activity-based probes (ABPs) that target active cathepsin X. These ABPs were used to label cathepsin X in complex lysates, whole cells, and in vivo. Furthermore, we have developed a method for selectively labeling and visualizing active cathepsin X in vitro and in vivo. Overall, the probes developed in this study are valuable tools for the study of cathepsin X function.
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