期刊
ACS CHEMICAL BIOLOGY
卷 4, 期 7, 页码 527-533出版社
AMER CHEMICAL SOC
DOI: 10.1021/cb900084v
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资金
- NCI NIH HHS [N01CO12400, N01 CO012400, N01-CO-12400] Funding Source: Medline
- NIAID NIH HHS [R01 AI072508-02, R01 AI072508-01, R01 AI072508] Funding Source: Medline
- NIDDK NIH HHS [P30 DK040561, P30 DK040561-14] Funding Source: Medline
The nematode Coenorhabditis elegons is a unique whole animal model system for identifying small molecules with in vivo anti-infective properties. C elegons can be infected with a broad range of human pathogens, including Enterococcus faecalis, an important human nosocomial pathogen. Here, we describe an automated, high-throughput screen of 37,200 compounds and natural product extracts for those that enhance survival of C elegans infected with E faecalis. Using a robot to dispense live, infected animals into 384-well plates and automated microscopy and image analysis, we identified 28 compounds and extracts not previously reported to have antimicrobial properties, including six structural classes that cure infected C elegons animals but do not affect the growth of the pathogen in vitro, thus acting by a mechanism of action distinct from antibiotics currently in clinical use.
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