期刊
IMMUNOLOGY
卷 145, 期 4, 页码 476-484出版社
WILEY
DOI: 10.1111/imm.12462
关键词
adenoviral vector; immunity; Middle East respiratory syndrome; coronavirus; spike protein; vaccine
类别
资金
- State Megaproject for Infectious Disease Research of China [2014ZX10004001-002, 2013ZX10004601, 2011ZX10004-803-006]
- National 973 Program of China [2011CB504704]
An ideal vaccine against mucosal pathogens such as Middle East respiratory syndrome coronavirus (MERS-CoV) should confer sustained, protective immunity at both systemic and mucosal levels. Here, we evaluated the in vivo systemic and mucosal antigen-specific immune responses induced by a single intramuscular or intragastric administration of recombinant adenoviral type 5 (Ad5) or type 41 (Ad41) -based vaccines expressing the MERS-CoV spike (S) protein. Intragastric administration of either Ad5-S or Ad41-S induced antigen-specific IgG and neutralizing antibody in serum; however, antigen-specific T-cell responses were not detected. In contrast, after a single intramuscular dose of Ad5-S or Ad41-S, functional antigen-specific T-cell responses were elicited in the spleen and pulmonary lymphocytes of the mice, which persisted for several months. Both rAd-based vaccines administered intramuscularly induced systemic humoral immune responses (neutralizing IgG antibodies). Our results show that a single dose of Ad5-S- or Ad41-S-based vaccines represents an appealing strategy for the control of MERS-CoV infection and transmission.
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