Enriching Mn-Doped ZnSe Quantum Dots onto Mesoporous Silica Nanoparticles for Enhanced Fluorescence/Magnetic Resonance Imaging Dual-Modal Bio-Imaging

Multimodal imaging is more suitable for disease diagnosis because of its ability to provide more complementary and accurate information over single-mode imaging. Mn-doped quantum dots (QDs), especially Mn-doped ZnS (ZnSe) QDs, possess unique fluorescent and magnetic properties and are thus attractive for fluorescence/magnetic resonance imaging (MRI) dual-mode imaging. However, the optimal dopant (Mn2+) concentration for maximizing the fluorescence of QDs is relatively low for the MRI imaging. Herein, based on the large Stokes shift of Mn-doped ZnSe QDs, an enrichment strategy with mesoporous silica (MSN) loading was explored for constructing a highly luminescent/paramagnetism Mn-doped ZnSe QDs assembly (MSN@QDs) for improved MRI/optical dual-model imaging. After assembly, the loading density of QDs in MSNs was estimated to be 152 +/- 12. Upon loading, the fluorescence of the MSN@QDs assembly was enriched along with QDs (enrichment factor of similar to 443). Because of the large Stokes shift (similar to 200 nm), no appreciable concentration quenching was observed. Meanwhile, the T-1 MR contrast was also increased both in vitro and in vivo through improved local Mn2+ concentration, realizing MRI signal enrichment. In fluorescence imaging investigations, MSN@QDs showed better performance over both single QDs and equivalent numbers of MSN-free single QD. Therefore, this enrichment strategy allowed simultaneous signal enhancement of the two imaging modes of Mn-doped ZnSe QDs.