期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 42, 页码 35838-35846出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b14717
关键词
near-infrared emission; iridium(III) complexes; dual-phosphorescent nanoprobe; elesclomol-treated tumors; hypochlorite
资金
- National Natural Science Foundation of China [51473078, 21671108]
- Natural Science Foundation of Jiangsu Province of China [BK20150833]
- National Program for Support of Top-Notch Young Professionals, Scientific and Technological Innovation Teams of Colleges and Universities in Jiangsu Province [TJ215006]
- Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University
- Priority Academic Program Development of Jiangsu Higher Education Institutions [YX03001]
Reactive oxygen species (ROS), when beyond the threshold, can exhaust the capacity of cellular antioxidants and ultimately trigger cell apoptosis in tumor biology. However, the roles of hypochlorite (CIO-) in this process are much less clear compared with those of ROS, and its detection is easily obstructed by tissue penetration and endogenous fluorophores. Herein, we first synthesized a near-infrared (NIR) ratiometric C1O(-) probe (Ir NP) composed of two kinds of phosphorescent iridium(III) complexes (Irl and Ir2) encapsulated with amphiphilic DSPE-mPEG5000. Ir NPs are dual-emissive and show obvious changes in phosphorescence intensity ratios and lifetimes of two emission bands upon exposure to CIO-. During the C1O(-) detection, ratiometric photoluminescence imaging is much more reliable over the intensity-based one for its self-calibration, while time-resolved photoluminescence imaging (TRPI) could distinguish the phosphorescence with long lifetime of Ir NPs from short-lived autofluorescence of tissues, resulting in the high accuracy of C1O(-) determination. With NIR emission, a long phosphorescence lifetime, fast response, and excellent biocompatibility, Ir NPs were applied to the detection of C1O(-) in vitro and in vivo by means of ratiometric phosphorescence imaging and TRPI with high signal-to noise-ratios (SNR). Importantly, we demonstrated the elevated C1O(-) in elesclomol-stimulated tumors in living mice for the first time, which holds great potential for the visualization of the boost of C1O(-) in anti-carcinogen-treated tumors and the further investigation of ROS-related oncotherapeutics.
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