期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 37, 页码 31088-31095出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b11365
关键词
diketopyrrolopyrrole; fluorescent probe; ratiometric; esterase; live and dead cells
资金
- National Natural Science Foundation of China [21772040, 21421004, 21372082, 21572062]
- Fundamental Research Funds for the Central Universities [222201717003]
- Programme of Introducing Talents of Discipline to Universities [B16017]
A new diketopyrrolopyrrole-based fluorescent probe (DPP-AM) was designed and synthesized for ratiometric detection of esterase and for imaging of live and dead cells in different modes. DPP-AM showed red fluorescence because of the intramolecular charge transfer (ICT) process from the DPP moiety to the pyridinium cation and gave remarkable ratio changes (about 70 folds), with the fluorescence changing from red to yellow, after treating with esterase because of the broken ICT process. Besides, the detection limit of DPP-AM toward esterase in vitro was 9.51 X 10(-5) U/mL. After pretreating with H2O2 and ultraviolet light radiation, the health status of TPC1 cells was successfully imaged. More importantly, DPP-AM showed yellow fluorescence in live cells and a red fluorescent signal in dead cells, indicating that assessing esterase activity as well as for discriminating live and dead cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据