期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 36, 页码 30662-30669出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b10007
关键词
quantum dot; graphene oxide; aptamer; supramolecular assembly; ratiometric fluorescence; lead ion; pH sensing
资金
- NSFC [21775021, 21545006, 21375015]
- priority discipline development program of Jiangsu province
The combination of functional nucleic acids and nanomaterials enables the continuous development of hybrid nanosystems that have found wide applications including chemo/biosensing. Herein, we report the supra molecular assembly of a sesame biscuit-like superstructural nanosystem based on aptamer, quantum dot (QD), and graphene oxide (GO), and its diverse applications in Pb2+ and pH sensing. The nanosystem was assembled via adsorbing silica-encapsulated green-emitting QD onto the edge of GO by ionic interaction, followed by absorbing aptamer-modified red-emitting QD onto the GO surface via the 3r-stacking interaction. The nanosystem showed the characteristic of the nonquenched green fluorescence due to silica encapsulation and quenched red fluorescence owing to nanomaterial surface energy transfer. The nanosystem responded to Pb2+/pH in ratiometric fluorescence: the red fluorescence varied upon analytedriven conformational changes of the aptamer, whereas the green one remained constant. Under optimized conditions, the nanosystem was demonstrated to be capable of quantifying Pb2+ with a detection limit of 11.7 pM, as well as pH with a sensing resolution of 0.1 pH unit. More importantly, the ratiometric nanosystem facilitated visualization of analytes in a distinct traffic light manner, which was exemplified by semiquantification of exogenous Pb2+ in living cells. To demonstrate practicality, fluorescent test strips were fabricated by immobilizing the nanosystem on paper. The fluorescent test strips displayed traffic light-type fluorescence color changes, with the capacity for on-site, naked-eye detection of Pb2+ in real samples, as well as point of-care pH testing in routine urinalysis.
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