期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 33, 页码 27612-27620出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b07671
关键词
metal-organic framework; boronic acid affinity; hydrophilicity; glycoprotein; enrichment
资金
- National Natural Science Foundation of China [21505112, 21775132]
- Scientific Research Foundation of Hunan Provincial Education Department [16A204]
- National Natural Science Foundation of Hunan province [2018JJ2388]
- Hunan 2011 Collaborative Innovation Center of Chemical Engineering & Technology with Environmental Benignity and Effective Resource Utilization
Protein glycosylation plays a critical role in post translational modifications of proteins in the organism and is involved in many diseases. However, the huge challenge for glycoproteins to be highly specific isolated and adsorbed from complicated biological samples results from their low abundance and interference. In this work, a novel dual-functionalized magnetic metal-organic frameworks nanoparticle for selective enrichment of glycoproteins was synthesized for the first time. Due to the abundant amino groups and grafted phenylboronic acid, the proposed nanoparticles have the dual properties of hydrophilicity and boronic acid affinity. The obtained nanoparticles show high binding capacities toward glycoproteins under physiological state (pH 7.4) such as ovalbumin (327.28 mg/g), transferrin (241.17 mg/g), horseradish peroxidase (530.79 mg/g). Furthermore, the nanoparticles still have excellent enrichment performance after being used six times repeatedly. More importantly, the prepared nanoparticles also have great potential applications in the adsorption of glycoproteins from complex biological specimens.
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