期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 1, 页码 713-720出版社
AMER CHEMICAL SOC
DOI: 10.1021/am507059n
关键词
microRNA; dual signal amplification; target catalyzed hairpin assembly; cascade catalysis
资金
- National Natural Science Foundation (NNSF) of China [21075100, 21275119, 51473136]
- Ministry of Education of China [708073]
- Specialized Research Fund of the Doctoral Program of Higher Education [20100182110015]
- Fundamental Research Funds for the Central Universities [XDJK2012A004, XDJK2013A008, XDJK2013A027]
In this work, a new electrochemical biosensor based on catalyzed hairpin assembly target recycling and cascade electrocatalysis (cytochrome c (Cyt c) and alcohol oxidase (AOx)) for signal amplification was constructed for highly sensitive detection of microRNA (miRNA). It is worth pointing out that target recycling was achieved only based on strand displacement process without the help of nuclease. Moreover, porous TiO2 nanosphere was synthesized, which could offer more surface area for Pt nanoparticles (PtNPs) enwrapping and enhance the amount of immobilized DNA strand 1 (S1) and Cyt c accordingly. With the mimicking sandwich-type reaction, the cascade catalysis amplification strategy was carried out by AOx catalyzing ethanol to acetaldehyde with the concomitant formation of high concentration of H2O2, which was further electrocatalyzed by PtNPs and Cyt c. This newly designed biosensor provided a sensitive detection of miRNA-155 from 0.8 fM to 1 nM with a relatively low detection limit of 0.35 fM.
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