期刊
ACS APPLIED MATERIALS & INTERFACES
卷 6, 期 19, 页码 17268-17276出版社
AMER CHEMICAL SOC
DOI: 10.1021/am505308f
关键词
graphene oxide; PEG; paclitaxel; A549; MCF-7
资金
- National Natural Science Foundation of China [21204098, 31100549, 11275050]
- Shanghai Scientific and Technological Innovation Project [11nm0501100, 11ZR1445900, 14520720100, 14520720700]
- State Key Laboratory of Bioreactor Engineering [2060204]
- Fundamental Research Funds for the Central Universities [222201313010]
Graphene oxide (GO), a novel 2D nanomaterial prepared by the oxidation of natural graphite, has been paid much attention in the area of drug delivery due to good biocompatibility and low toxicity. In the present work, 6-armed poly(ethylene glycol) was covalently introduced into the surface of GO sheets via a facile amidation process under mild conditions, making the modified GO, GO-PEG (PEG: 65 wt %, size: 50-200 nm), stable and biocompatible in physiological solution. This nanosized GO-PEG was found to be nontoxic to human lung cancer A549 and human breast cancer MCF-7 cells via cell viability assay. Furthermore, paclitaxel (PTX), a widely used cancer chemotherapy drug, was conjugated onto GO-PEG via pi-pi stacking and hydrophobic interactions to afford a nanocomplex of GO-PEG/PTX with a relatively high loading capacity for PTX (11.2 wt %). This complex could quickly enter into A549 and MCF-7 cells evidenced by inverted fluorescence microscopy using Fluorescein isothiocyanate as a probe, and it also showed remarkably high cytotoxicity to A549 and MCF-7 cells in a broad range of concentration of PTX and time compared to free PTX. This kind of nanoscale drug delivery system on the basis of PEGylated GO may find potential application in biomedicine.
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