期刊
ACS APPLIED MATERIALS & INTERFACES
卷 6, 期 3, 页码 1971-1978出版社
AMER CHEMICAL SOC
DOI: 10.1021/am405017h
关键词
cycloolefin polymer (COP); surface-initiated photoiniferter-mediated polymerization (SI-PIMP); hierarchical architecture; antibody immobilization; immunoassay
资金
- National Natural Science Foundation of China [21274150, 51273200]
- Chinese Academy of Sciences-Wego Group High-tech Research & Development Program
- Scientific Development Program of Jilin Province [20130102064JC]
The development of technologies for a biomedical detection platform is critical to meet the global challenges of various disease diagnoses. In this study, an inert cycloolefin polymer (COP) support was modified with two-layer polymer brushes possessing dual functions, i.e., a low fouling poly[poly(ethylene glycol) methacrylate] [p-(PEGMA)] bottom layer and a poly(acrylic acid) (PAA) upper layer for antibody loading, via a surface-initiated photoiniferter-mediated polymerization strategy for fluorescence-based immunoassay. It was demonstrated through a confocal laser scanner that, for the as-prepared COP-g-PEG-b-PAA-IgG supports, nonspecific protein adsorption was suppressed, and the resistance to nonspecific protein interference on antigen recognition was significantly improved, relative to the COP-g-PAA-IgG references. This strategy for surface modification of a polymeric platform is also applicable to the fabrication of other biosensors.
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