4.8 Article

Substrate Stiffness Together with Soluble Factors Affects Chondrocyte Mechanoresponses

期刊

ACS APPLIED MATERIALS & INTERFACES
卷 6, 期 18, 页码 16106-16116

出版社

AMER CHEMICAL SOC
DOI: 10.1021/am504135b

关键词

substrate stiffness; chondroryte; actin; focal adhesion; traction force; stretch

资金

  1. National Natural Science Foundation of China [31130021, 31300773]
  2. China Postdoc Grant [2013M532152]
  3. Chongqing Postdoc Grant [Xm201352]

向作者/读者索取更多资源

Tissue cells sense and respond to differences in substrate stiffness. In chondrocytes, it has been shown that substrate stiffness regulates cell spreading, proliferation, chondrogenic gene expression, and TGF-beta signaling. But how the substrate stiffness together with soluble factors influences the mechanical properties of chondrocyte is still unclear. In this study, we cultured goat articular chondrocytes on polyacrylamide gels of 1, 11, and 90 kPa (Young's modulus), and measured cellular stiffness, traction force, and response to stretch in the presence of TGF-beta 1 or IL-1 beta. We found that TGF-beta 1 increased cellular stiffness and traction force and enhanced the response to stretch, while IL-1 beta increased cellular stiffness, but lowered traction force and weakened the response to stretch. Importantly, the effects of TGF-beta 1 on chondrocyte mechanics were potent in cells cultured on 90 kPa substrates, while the effects of IL-1 beta were potent on 1 kPa substrates. We also demonstrated that such changes of chondrocyte mechanoresponse were due to not only the changes of actin cytoskeleton and focal adhesion, but also the alteration of chondrocyte extracellular matrix synthesis. Taken together, these results provide insights into how chondrocytes integrate physical and biochemical cues to regulate their biomechanical behavior, and thus have implications for the design of optimized mechanical and biochemical microenvironments for engineered cartilage.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据