期刊
ACS APPLIED MATERIALS & INTERFACES
卷 6, 期 8, 页码 5467-5471出版社
AMER CHEMICAL SOC
DOI: 10.1021/am500412v
关键词
HOMMS@TiO2; selective enrichment; phosphoproteomics; mass spectrometry
资金
- 973 program [2012B910602, 2013CB911201]
- NSFC program [21075022, 20875017, 21105016]
Metal oxide affinity chromatography (MOAC) is a powerful technique in phosphoproteome research. However, the achievement of highly specific enrichment and sensitive detection of phosphopeptide by MOAC remains a big challenge since the lack of high specificity and large binding capacity of conventional MOAC materials. In this work, a new MOAC material, TiO2-coated hierarchically ordered macro/mesoporous silica (denoted as HOMMS@TiO2) composites, was prepared via a facile process. The HOMMS@TiO2 composites were demonstrated to have low limit of detection (8 fmol) and great specificity with a very rapid enrichment speed (within 1 min). These experimental results have demonstrated that the HOMMS@TiO2 exhibit great potential in phosphoproteome research.
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