期刊
ACS APPLIED MATERIALS & INTERFACES
卷 6, 期 18, 页码 16197-16203出版社
AMER CHEMICAL SOC
DOI: 10.1021/am5043164
关键词
quantum dots; graphene oxide; photoelectrochemistry; enzyme catalysis; biosensing
资金
- National Natural Science Foundation of China [21475062, 21175069, 21205061]
- Natural Science Foundation of Jiangsu [BK2012448]
- Foundation of the Jiangsu Education Committee [11KJA150003]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
An innovative photoelectrochemical (PEC) biosensor platform was designed based on the in situ generation of CdS quantum dots (QDs) on graphene oxide (GO) using an enzymatic reaction. Horseradish peroxidase catalyzed the reduction of sodium thiosulfate with hydrogen peroxide to generate H2S, which reacted with Cd2+ to form CdS QDs. CdS QDs could be photoexcited to generate an elevated photocurrent as a readout signal. This strategy offered a green alternative to inconvenient presynthesis procedures for the fabrication of semiconducting nanoparticles. The nanomaterials and assembly procedures were characterized by microscopy and spectroscopy techniques. Combined with immune recognition and on the basis of the PEC activity of CdS QDs on GO, the strategy was successfully applied to a PEC assay to detect carcinoembryonic antigen and displayed a wide linear range from 2.5 ng mL(-1) to 50 mu g mL(-1) and a detection limit of 0.72 ng mL(-1) at a signal-to-noise ratio of 3. The PEC biosensor showed satisfactory performance for clinical sample detection and was convenient for determining high concentrations of solute without dilution. This effort offers a new opportunity for the development of numerous rapid and convenient analytical techniques using the PEC method that may be applied in the design and preparation of various solar-energy-driven applications.
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