期刊
ACS APPLIED MATERIALS & INTERFACES
卷 6, 期 14, 页码 11799-11804出版社
AMER CHEMICAL SOC
DOI: 10.1021/am502529a
关键词
magnetic graphene; mesoporous silica; size-exclusive; titanium; endogenous phosphorylated peptides
资金
- 973 Project [2013CB911201, 2012CB910602]
- 863 Project [2012AA020202]
- National Natural Science Foundation of China [21075022, 20875017, 21105016]
Developing an effective separation method is necessary for identifying low-abundant endogenous phosphorylated peptides with the removal of proteins. In this work, we prepared size-exclusive magnetic graphene/mesoporous silica composites with titanium(lV)-immobilized pore walls (denoted as Ti4+-MGMSs) for capturing endogenous phosphorylated peptides for mass spectrometry analysis. The introduction of hydrophilic polydopamine simplified the synthetic process of Ti4+-MGMSs, and the ordered mesoporous channels are beneficial to the trapping of endogenous phosphopeptides while large-size proteins are excluded. Furthermore, the magnetic performance greatly simplifies the entire process of enrichment. With all of the advances, the novel Ti4+-MGMSs present high enrichment efficiency either from the low concentration of beta-casein tryptic digest (0.5 fmol/mu L) or the mixture of beta-casein tryptic digest and alpha-casein (or plus bovine serum albumin, with a mass ratio up to 1:500). Besides, Ti4+-MGMSs have also been successfully applied to enrich endogenous phosphorylated peptides from human serum and human saliva.
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