期刊
ACS APPLIED MATERIALS & INTERFACES
卷 5, 期 13, 页码 5947-5953出版社
AMER CHEMICAL SOC
DOI: 10.1021/am402115v
关键词
electrospinning; layer-by-layer; poly(beta-amino ester); biodegradable; gene delivery
资金
- National Institutes of Health [1R01EB016721-01]
- Global-COE program (Kyushu University, Japan) foundation
- National Science Foundation (Arlington, VA, USA)
Biodegradable polyelectrolyte surfaces for gene delivery were created through electrospinning of biodegradable polycations combined with iterative solution-based multilayer coating. Poly(beta-amino ester) (PBAE) poly(1,4-butanediol diacrylate-co-4-amino-1-butanol) end-capped with 1-(3-aminopropyl)-4-methylpiperazine was utilized because of its ability to electrostatically interact with anionic molecules like DNA, its biodegradability, and its low cytotoxicity. A new DNA release system was developed for sustained release of DNA over 24 h, accompanied by high exogenous gene expression in primary human glioblastoma (dB) cells. Electrospinning a different PBAE, poly(1,4-butanediol diacrylate-co-4,4'-trimethylenedipiperidine), and its combination with polyelectrolyte 1-(3-aminopropyl)-4-methylpiperazine end-capped poly(1,4-butanediol diacrylate-co-4-amino-1-butanol)-based multilayers are promising for DNA release and intracellular delivery from a surface.
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