期刊
ACS APPLIED MATERIALS & INTERFACES
卷 5, 期 3, 页码 532-537出版社
AMER CHEMICAL SOC
DOI: 10.1021/am302580p
关键词
enzyme; enzyme assisted biomineralization; bioinorganic nanocomposites; enzyme reuse; amorphous calcium phosphate; functional nanocomposites
资金
- National Research Foundation of Korea
- Korean Government (MEST) [NRF-C1ABA001-2010-0020501]
In the present study, we report on enzyme-assisted formation of biomineralized amorphous calcium phosphate nanocomposites (ACP-NCs). About 100-200 nm sizes of the spherical porous enzyme-assisted ACP-NCs were successfully synthesized via double reverse microemulsion, but no ACP-NCs formed without the enzyme. It is believed that the enzyme was used as an organic template or additive that could regulate the biomineralization process. The enzyme-assisted ACP-NCs were well characterized by X-ray diffraction, transmission electron microscopy, scanning electron microscopy, dynamic light scattering, and Brunauer-Emmett-Teller (BET) criteria. The BET surface area, total pore volume, pore size from adsorption, and pore size from desorption of the ACP-NCs were 163 m(2) g(-1) or 0.37 cm(3) g(-1), 8.87 nm, and 7.48 nm, respectively. The enzyme-assisted ACP-NCs retained about 43% of the catalytic activity of free carboxyl esterase. Furthermore, they preserved their bioactivity even after the 10th reuse and were stable over 10 days even under a stringent shaking conditions. The reported method paves the way for novel biomineralization via enzyme molecules to form functional enzymes containing nanocomposites.
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