期刊
ACS APPLIED MATERIALS & INTERFACES
卷 5, 期 17, 页码 8351-8358出版社
AMER CHEMICAL SOC
DOI: 10.1021/am401285x
关键词
magnetic particles; composite microspheres; core-shell; boronic acid; enrichment; glycoprotein
资金
- National Science Foundation of China [20874015, 51273047]
- Shanghai Rising-Star Program [10QH1400200]
- Shu Guang project [12SG07]
- Shanghai Municipal Education Commission
- Shanghai Education Development Foundation
In this work, core shell shell-structured boronic acid-functionalized magnetic composite microspheres Fe3O4@SiO2@poly (methyl methacrylate-co-4-vinylphenylbornoic acid) (Fe3O4@SiO2@P(MMA-co-VPBA)) with a uniform size and fine morphology were synthesized. Here, Fe3O4 magnetic particles were prepared by a solvothermal reaction, whereas the Fe3O4@SiO2 microspheres with a core shell structure were obtained by a sol gel process. 3-(Trimethoxysilyl) propyl methacrylate (MPS)-modified Fe3O4@SiO2 was used as the seed in the emulsion polymerization of MMA and VPBA to form the core shell shell-structured magnetic composite microspheres. As the boronic acid groups on the surface of Fe3O4@SiO2@P(MMA-co-VPBA) could form tight yet reversible covalent bonds with the cis-1,2-diols groups of glycoproteins, the magnetic composite microspheres were applied to enrich a standard glycoprotein, horseradish peroxidase (HRP), and the results demonstrated that the composite microspheres have a higher affinity for the glycoproteins in the presence of the nonglycoprotein bovine serum albumin (BSA) over HRP. Additionally, different monomer mole ratios of MMA/VPBA were studied, and the results implied that using MMA as the major monomer could reduce the amount of VPBA with a similar glycoprotein enrichment efficiency but a lower cost.
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