期刊
ACS APPLIED MATERIALS & INTERFACES
卷 5, 期 10, 页码 4076-4085出版社
AMER CHEMICAL SOC
DOI: 10.1021/am3028537
关键词
gold nanorods; peptide; stability; cell viability; Alzheimer's disease; amyloidogenesis; NIR irradiation
资金
- AECID [Fondecyt 1090143, Fondecyt 1130425, Anillo ACT-95, MECESUP-0811]
- CICYT [CTQ2012-30930]
- Generalitat de Catalunya [2009SGR 1024]
- Institute for Research in Biomedicine
- Barcelona Science Park
- MECESUP
- CONICYT [Fondecyt 3130654]
- Vicerrectoria USACH
- ICREA Funding Source: Custom
Gold nanorods used in therapy and diagnosis must be nontoxic and stable in biological media and should be specific for the target. The complete combination of these three factors has hindered the use of gold nanorods as carriers in biological and biomedical applications. In this study, we produced a conjugate of gold nanorods with the peptide CLPFFD that recognizes toxic beta-amyloid aggregates present in Alzheimer's disease, demonstrates colloidal stability, maintains plasmonic properties, and shows no effects on cell viability in the SH-SY5Y cell line. Furthermore, the irradiation of beta-amyloid in the presence of the conjugate with near-infrared region irradiation energy reduces the amyloidogenic process reducing also its cytotoxicity. The nanorods were synthesized following the seed-mediated method in cetyltrimethylammonium bromide (CTAB) and were conjugated with the N-terminal cysteine peptide, CLPFFD. The conjugate was exhaustively characterized using different techniques (Absorption spectroscopy, X-ray photoelectron spectroscopy, electron energy loss spectroscopy, and zeta potential). The effects on cell viability and cell penetration by transmission electron microscopy of the conjugate were evaluated. The chemisorption of the peptide on the surface of gold nanorods increases their stability and reduces their effects on cell viability.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据