期刊
ACS APPLIED MATERIALS & INTERFACES
卷 4, 期 9, 页码 4524-4531出版社
AMER CHEMICAL SOC
DOI: 10.1021/am3007565
关键词
layer-by-layer; graphene; polyelectrolyte multilayers; nanofibers; scaffolds; neural tissue engineering
资金
- ARC Special Initiative in bionic vision science technology
- Contributing to Australian Scholarship and Science (CASS) Foundation
- Eva and Les Erdi AUSiMED Fellowship in Neurological Diseases
- Diana Asmar Fund
- National Health and Medical Research Council of Australia
- ARC Discovery [DP0985433]
- Australian Research Council [DP0985433] Funding Source: Australian Research Council
Electroactive scaffolds that are passively conductive and able to transmit applied electrical stimuli are of increasing importance for neural tissue engineering. Here, we report a process of rendering both 2D and 3D polymer scaffolds electrically conducting, while also enhancing neuron attachment. Graphene-heparin/poly-L-lysine polyelectrolytes were assembled via layer-by-layer (LbL) deposition onto 2D surfaces and 3D electrospun nanofibers. The employed LbL coating technique in this work enables the electro- and biofunctionalization of complex 3D scaffold structures. LbL assembly was characterized by a steady mass increase during the in situ deposition process in 2D, with regular step changes in hydrophobicity. Uniform coverage of the graphene/polyelectrolyte coatings was also achieved on nanofibers, with hydrodynamic flow and post-thermal annealing playing an important role in controlling sheet resistance of 2D surfaces and nanofibers. Cell culture experiments showed that both 2D and 3D graphene-PEMs supported neuron cell adhesion and neurite outgrowth, with no appreciable cell death. This electroactive scaffold modification may therefore assist in neuronal regeneration, for creating functional and biocompatible polymer scaffolds for electrical entrainment or biosensing applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据