期刊
ACS APPLIED MATERIALS & INTERFACES
卷 4, 期 4, 页码 1963-1971出版社
AMER CHEMICAL SOC
DOI: 10.1021/am300391j
关键词
electrospun PVA nanofibers; cross-link; water stable; Au nanoparticles; H2O2 detection; biosensors
资金
- National Basic Research Program of China [2010CB934700]
- National Natural Science Foundation of China [91022032, 21061160492, J1030412]
- International Science & Technology Cooperation Program of China [2010DFA41170]
- National Synchrotron Radiation Laboratory at the University
Electrospun nanofibrous mats are intensively studied as efficient scaffold materials applied in the fields of tissue engineering, catalysis, and biosensors due to their flexibility and porosity. In this paper, we report a facile route to fabricate gold nanoparticles-poly(vinyl alcohol) (Au NPs-PVA) hybrid water stable nanofibrous mats with tunable densities of Au NPs and further demonstrate the potential application of as-prepared Au NPs-PVA nanofibrous mats as efficient biosensor substrate materials. First, through the designed in situ cross-linkage in coelectrospun PVA-glutaraldehyde nanofibers, water insoluble PVA nanofibrous mats with suitable tensile strength were successfully prepared. Then, 3-mercaptopropyltrimethoxysilane (MPTES) was modified on the surface of obtained PVA nanofibrous films, which triggered successful homogeneous decoration of Au NPs through gold sulfur bonding interactions. Finally, the Au NPs-PVA nanofibrous mats embedded with horseradish peroxidase (HRP) by electrostatic interactions were used as biosensor substrate materials for H2O2 detection. The fabricated HRP-Au NPs/PVA biosensor showed a highly sensitive detection of H2O2 with a detection limit of 0.5 mu M at a signal-to-noise ratio of 3. By modifying other different functional nanaoparticles or enzyme on the PVA nanofibrous film will further expand their potential applications as substrate materials of different biosensors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据