期刊
IMMUNOBIOLOGY
卷 220, 期 10, 页码 1177-1185出版社
ELSEVIER GMBH
DOI: 10.1016/j.imbio.2015.06.001
关键词
Ficolin; Complement system; Lectin pathway; Bacteria; Innate immunity
类别
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2010/50043-0]
- Conselho Nacional de Pesquisa e Desenvolvimento (CNPq) from Brazil
- Universidad Nacional Autonoma de Mexico (UNAM)
- Svend Andersen Research Foundation
- Novo Nordisk Research Foundation
- Research Foundation of the Capital Region of Denmark
- Research Foundation at Rigshospitalet
- Danish Medical Research Council
Ficolins recognize pathogen associated molecular patterns and activate the lectin pathway of complement system. However, our knowledge regarding pathogen recognition of human ficolins is still limited. We therefore set out to explore and investigate the possible interactions of the two main serum ficolins, ficolin-2 and ficolin-3 with different Gram-negative bacteria. We used recombinant ficolin molecules and normal human serum, which were detected with anti-ficolin monoclonal antibodies. In addition we investigated the capacity of these pathogens to activate the lectin pathway of complement system. We show for the first time that human ficolin-2 recognizes the nonpathogenic spirochete Leptospira biflexa serovar Patoc, but not the pathogenic Leptospira interrogans serovar Kennewicki strain Fromm. Additionally, human ficolin-2 and ficolin-3 recognize pathogenic Pasteurella pneumotropica, enteropathogenic Escherichia coli (EPEC) serotype O111ab:H2 and enteroaggregative E. coli (EAEC) serogroup O71 but not four enterohemorrhagic E. coli, three EPEC, three EAEC and two nonpathogenic E. coli strains (DH5 alpha and HB101). The lectin pathway was activated by Pasteurelia pneumotropica, EPEC O111ab:H2 and EAEC 071 after incubation with Clq depleted human serum. In conclusion, this study provide novel insight in the binding and complement activating capacity of the lectin pathway initiation molecules ficolin-2 and ficolin-3 towards relevant Gram-negative pathogens of pathophysiological relevance. (C) 2015 Elsevier GmbH. All rights reserved.
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