期刊
ACS APPLIED MATERIALS & INTERFACES
卷 3, 期 4, 页码 1306-1310出版社
AMER CHEMICAL SOC
DOI: 10.1021/am200101n
关键词
acetylcholinesterase; myristoylcholine; polythiophene; biosensor; aggregation
资金
- National Natural Science Foundation of China [50973054, 50873092]
A complex between an anionic polythiophene derivative (PT-COO-) and a cationic surfactant, myristoylcholine, has been prepared and applied to be colorimetric probe for acetylcholinesterase (AChE) assays. The complex formation process, AChE activity assay and inhibitor screening has been studied by absorption spectroscopy. It was confirmed that the introduction of myristoylcholine into PT-COO- phosphate buffer solution resulted in the disassembly of PT-COO- aggregates, and further addition of AChE into the above solution led to the reassembly of PT-COO- due to the catalyzed hydrolysis of myristoylcholine and the collapse of the complex. The colorimetric assay for AChE can be readily realized with the concentration of AChE as low as 0.2 U/mL. The results also demonstrate that the colorimetric approach can be applied for screening inhibitors of AChE.
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