期刊
IET NANOBIOTECHNOLOGY
卷 9, 期 5, 页码 264-272出版社
INST ENGINEERING TECHNOLOGY-IET
DOI: 10.1049/iet-nbt.2015.0008
关键词
enzymes; molecular biophysics; silver; nanoparticles; nanocomposites; nanomedicine; cellular biophysics; colloids; cancer; scanning electron microscopy; transmission electron microscopy; ultraviolet spectra; visible spectra; particle size; biochemistry; drugs; drug delivery systems; biomedical materials; plumbagin-silver nanoparticle formulations; cellular uptake; antiproliferative activity; colloidal silver nanoparticles; drug delivery system; cancer medicine; nontoxic napthaquinone; plumbago indica; human cervical cancer cell line; cytotoxic activity; chemical reduction method; dynamic light scattering; high-resolution scanning electron microscopy; transmission electron microscopy; ultra-violetvisible spectrophotometer; cell inhibition; sulphorhodamine B assay; mitotic index; Wright-Giemsa staining; apoptosis induction; western blot; cleaved polyadenosine diphosphate-ribose polymerase antibody; particle size; enhanced internalisation; HeLa cells; PLB inhibited cell proliferation; cell mitosis; post-drug exposure clonogenic cell survival; antiproliferative activities; antimitotic activities; apoptotic activities; cancer treatment; size 8 nm to 32 nm
资金
- [NITC] National Institute of Technology Calicut [(CSR)/FRG10/0109]
- Department of Biotechnology, Government of India
Colloidal silver nanoparticles (AgNPs) have attracted much attention in recent years as diagnostics and new drug delivery system in cancer medicine. To study the effects of plumbagin (PLB), a relatively non-toxic napthaquinone isolated from the roots of Plumbago indica in human cervical cancer cell line and developed a formulation to enhance its cytotoxic activities. Silver nanoparticles were synthesised by chemical reduction method and complexed with PLB. Both the AgNPs and the complex PLB-AgNPs were characterised by dynamic light scattering, high-resolution scanning electron microscopy and transmission electron microscopy. The amount of PLB and PLB-AgNPs internalised was determined by ultra-violet-visible spectrophotometer. Cell inhibition was determined by sulphorhodamine B assay. Mitotic index was determined by Wright-Giemsa staining. Apoptosis induction was assessed by western blot using cleaved poly adenosine diphosphate-ribose polymerase antibody. The scanning electron microscope analysis indicated an average particle size of 32 +/- 8 nm in diameter. Enhanced internalisation of PLB into the HeLa cells was observed in PLB-AgNPs. PLB inhibited proliferation of cells with IC50 value of about 18 +/- 0.6 mu M and blocked the cells at mitosis in a concentration-dependent manner. PLB also inhibited the post-drug exposure clonogenic survival of cells and induced apoptosis. The antiproliferative, antimitotic and apoptotic activities were also found to be increased when cells were treated with PLB-AgNPs. The authors results support the idea that AgNP could be a promising and effective drug delivery system for enhanced activity of PLB in cancer treatment.
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