期刊
JOURNAL OF ACUPUNCTURE AND MERIDIAN STUDIES
卷 4, 期 3, 页码 193-197出版社
ELSEVIER
DOI: 10.1016/j.jams.2011.09.008
关键词
antigenotoxicity; comet assay; DNA damage; oxidative stress; portulaca oleracea; single-cell gel electrophoresis
资金
- Research Committee of Pharmacy School
- Research Council of Mashhad University of Medical Sciences, Iran
The comet assay is a standard method for measuring DNA damage. In this study, the protective effects of ethanolic and aqueous extracts of Portulaca oleracea L. (P. oleracea) on human lymphocyte DNA lesions were evaluated with the comet assay. Lymphocytes were isolated from blood samples taken from healthy volunteers. Human lymphocytes were incubated in H2O2 (50,100, and 200 mu M), aqueous extract (0.05, 0.1, 0.5, 1, and 2.5 mg/ml), and ethanolic extracts (0.05, 0.1, 0.5, 1, and 2.5 mg/ml) of P. oleraceae aerial parts alone with a combination of H2O2 (100 mM) with either 1 or 2.5 mg/ml of both extracts at 4 degrees C for 30 minutes. The extent of DNA migration was measured using the alkaline single cell gel electrophoresis approach assay, and DNA damage was expressed as percentage tail DNA. We found that the aqueous extract of P. oleracea significantly inhibited DNA damage, while there was no effect of the ethanolic extract. These data suggest that the aqueous extract of P. oleracea can prevent oxidative DNA damage to human lymphocytes, which is likely due to antioxidant constituents in the extract.
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