期刊
AAPS PHARMSCITECH
卷 13, 期 3, 页码 919-927出版社
SPRINGER
DOI: 10.1208/s12249-012-9816-1
关键词
drug delivery; particle size distribution; pH nanoprecipitation; protein polymers; zein; zeta potential
资金
- South Dakota State Corn Utilization Council
- Governor's 2010 competitive research seed grant
- Department of Pharmaceutical Sciences, South Dakota State University
The main objective of the present study was to investigate the influence of various formulation parameters on the preparation of zein nanoparticles. 6,7-dihydroxycoumarin (DHC) was used as a model hydrophobic compound. The influence of pH of the aqueous phase, buffer type, ionic strength, surfactant, and zein concentration on particle size, polydispersity index, and zeta potential of DHC-loaded zein nanoparticles were studied. Smaller nanoparticles were formed when the pH was close to the isoelectric point of zein. DHC-loaded zein nanoparticles prepared using citrate buffer (pH 7.4) was better than phosphate buffer in preventing particle aggregation during lyophilization. The ionic strength did not have a significant influence on the particle size of DHC-loaded zein nanoparticles. A combination of Pluronic F68 and lecithin in 2:1 ratio stabilized the zein nanoparticles. An increase in zein concentration led to increase in particle size of DHC-loaded zein nanoparticles. The use of optimal conditions produced DHC-loaded nanoparticles of 256 +/- 30 nm and an encapsulation efficiency of 78 +/- 7%. Overall, the study demonstrated the optimal conditions to prepare zein nanoparticles for drug encapsulation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据