期刊
ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES
卷 13, 期 5, 页码 498-504出版社
SHENYANG PHARMACEUTICAL UNIV
DOI: 10.1016/j.ajps.2018.05.006
关键词
Hyaluronic acid; QCD-g-CS; Polymeric nanoparticles; Ionic gelation; Delivery system
资金
- Mae Fah Luang University
- Thailand Graduate Institute of Science and Technology (TGIST), National Science and Technology Development Agency (NSTDA), Thailand [SCA-CO-2558-1026-TH]
Hyaluronic acid (HA, 20-50 kDa) is a hydrophilic macromolecule with anti-wrinkle effects and moisturizing properties. However, its high molecular weight prevents it from penetrating into the deeper layers of the skin and, thus, limits its benefits to topical effects. Thus, the objective of this study is to prepare nanoparticles of quaternized cyclodextrin-grafted chitosan (QCD-g-CS) associated with HA in different molar ratios of QCD-g-CS and HA. The conjugation of the carboxylic moieties of HA and the amides of QCD-g-CS was confirmed by Fourier-transform infrared spectroscopy. Thus, the system was optimized to create nanoparticles with a small size (235.63 +/- 21.89 nm), narrow polydispersity index (0.13 +/- 0.02), and zeta potential of 16.07 +/- 0.65 mV. The association efficiency and loading efficiency were determined by ultra-performance liquid chromatography as 86.77 +/- 0.69% and 10.85 +/- 0.09%, respectively. The spherical morphology of the obtained nanoparticles was confirmed by transmission electron microscopy. Moreover, the in-vitro hydrating ability was significantly higher (P < 0.001) than that of bulk HA (3.29 +/- 0.41 and 1.71 +/- 0.05 g water/g sample, respectively). The safety of these nanoparticles at concentrations in the range of 0.01-0.10 mg/ml was confirmed via tests on human skin fibroblasts. Together, these results demonstrate that the developed nanoparticles are promising for future applications in cosmetics. (C) 2018 Shenyang Pharmaceutical University. Published by Elsevier B.V.
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