期刊
ACS SENSORS
卷 3, 期 2, 页码 320-326出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.7b00720
关键词
IFN-gamma detection; AIE fluorogens; aptasensor; bioimaging; turn-on fluorescence
资金
- National Basic Research Program of China [2013CB834701]
- China Scholarship Council [201506170099]
- National Natural Science Foundation of China [21674041, 215750045, 51373063]
- ARC Centre of Excellence for Nanoscale BioPhotonics [CE140100003]
- Macquarie University MQRDG
- ARC Future Fellowship
We report an aggregation-induced emission fluorogen (AIEgen)-based turn-on fluorescent aptasensor able to detect the ultrasmall concentration of intracellular IFN-gamma. The aptasensor consists of an IFN-gamma aptamer labeled with a fluorogen with a typical aggregation-induced emission (AIE) characteristic, which shows strong red emission only in the presence of IFN-gamma. The aptasensor is able to effectively monitor intracellular IFN-gamma secretion with the lowest detection limit of 2 pg and it is capable of localizing IFN-gamma in live cells during secretion, with excellent cellular permeability and biocompatibility as well as low cytotoxicity. This probe is able to localize the intracellular IFN-gamma at a low concentration <10 pg mL(-1), and it is successfully used for real-time bioimaging. This simple and highly sensitive sensor may enable the exploration of cytokine pathways and their dynamic secretion process in the cellular environment. It provides a universal sensing platform for monitoring a spectrum of molecules secreted by cells.
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