期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 4, 期 4, 页码 1471-1478出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.7b00944
关键词
nanoparticle reactors; nanoerythrosomes; colloidal nanoparticles; enzyme reaction; self-assembled membrane vesicles
资金
- FAR, MIUR grant, University of Chieti-Pescara G. d'Annunzio, Chieti, Italy
- Tekes large strategic research opening 3i Regeneration [40395/13]
- Academy of Finland [297580]
- Jane and Aatos Erkko Foundation [4704010]
- Sigrid Juselius Foundation [28001830K1, 4704580]
- University of Helsinki Research Funds
- HiLIFE Research Funds
- European Research Council under the European Union's Seventh Framework Programme (FP) [310892]
Organelles of eukaryotic cells are structures made up of membranes, which carry out a majority of functions necessary for the surviving of the cell itself. Organelles also differentiate the prokaryotic and eukaryotic cells, and are arranged to form different compartments guaranteeing the activities for which eukaryotic cells are programmed. Cell membranes, containing organelles, are isolated from cancer cells and erythrocytes and used to form biocompatible and long circulating ghost nanoparticles delivering payloads or catalyzing enzymatic reactions as nanoreactors. In this attempt, red blood cell membranes were isolated from erythrocytes, and engineered to form nanoerythrosomes (NERs) of 150 nm. The horseradish peroxidase, used as an enzyme model, was loaded inside the aqueous compartment of NERs, and its catalytic reaction with Resorufm was monitored. The resulting nanoreactor protected the enzyme from proteolytic degradation, and potentiated the enzymatic reaction in situ as demonstrated by maximal velocity (V-max) and Michaelis constant (K-m), thus suggesting the high catalytic activity of nanoreactors compared to the pure enzymes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据