期刊
CELL SYSTEMS
卷 6, 期 1, 页码 25-+出版社
CELL PRESS
DOI: 10.1016/j.cels.2017.12.001
关键词
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资金
- SNSF R'Equip grant
- SNSF Assistant Professorship grant
- SystemsX Transfer Project Friends and Foes''
- SystemsX MetastasiX and PhosphoNetX grant
- NIH [UC4 DK108132]
- European Research Council (ERC) under the European Union/ERC [336921]
- EMBO fellowship [ALTF 970-2014]
- European Commission (LTFCOFUND) [GA-2013-609409]
- Forschungskredit of the University of Zurich [FK-17-115, 74419-01-01]
- University of Zurich [BIOEF-17-001]
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [UC4DK108132] Funding Source: NIH RePORTER
To build comprehensive models of cellular states and interactions in normal and diseased tissue, genetic and proteomic information must be extracted with single-cell and spatial resolution. Here, we extended imaging mass cytometry to enable multiplexed detection of mRNA and proteins in tissues. Three mRNA target species were detected by RNAscope-based metal in situ hybridization with simultaneous antibody detection of 16 proteins. Analysis of 70 breast cancer samples showed that HER2 and CK19 mRNA and protein levels are moderately correlated on the single-cell level, but that only HER2, and not CK19, has strong mRNA-to-protein correlation on the cell population level. The chemoattractant CXCL10 was expressed in stromal cell clusters, and the frequency of CXCL10-expressing cells correlated with T cell presence. Our flexible and expandable method will allow an increase in the information content retrieved from patient samples for biomedical purposes, enable detailed studies of tumor biology, and serve as a tool to bridge comprehensive genomic and proteomic tissue analysis.
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