期刊
JOURNAL OF INFECTION AND PUBLIC HEALTH
卷 11, 期 1, 页码 64-68出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.jiph.2017.03.010
关键词
OXA-48; NDM; Carbapenem resistance; Saudi Arabia
资金
- Deanship of Scientific Research at King Saud University [RGP-038]
The main objective of this work was to characterize carbapenemases, extended-spectrum ?-lactamases (ESBLs), and plasmid-mediated quinolone resistance (PMQR) among carbapenem-insensitive Klebsiella pneumoniae and Escherichia coli clinical isolates which were isolated from three hospitals in Riyadh. Thirty-one carbapenem-insensitive isolates (21 K. pneumoniae and 10 E. coli) were recovered from March 2014 to May 2014. Susceptibility testing and phenotypic detection tests were used to characterize the classes of ?-lactamases. PCR assays were performed for the detection of the genes encoding ESBL (bla(CTX-M), bla(TEM),bla(SHV), and bla(OXA-1)), carbapenemase (bla(KPC),bla(GES),bla(VIM), bla(IMP), bla(NDM), and bla(OXA-48)), and PMQR (qnrA, qnrB, qnrS, aac(6)-Ib-cr, qepA, oqxA, and oqxB) genes. All carbapenem-insensitive isolates were carbapenemase producers, with 41.9% and 58.1% being class B carbapenemases class D OXA-48, respectively. While the prevalence of ESBL producers was 80.6%. The following resistance genes were detected; OXA-48-like (58.1%), NDM-type (41.9%), CTX-M-1-like (77.4%), CTX-M-9-like (9.6%), TEM-1 (74.2%), OXA-1 (54.8%), SHV-1 (4.4%), qnrS (58.1%), qnrB (3.2%), and aac(6)-Ib-cr (51.6%). The predominant carbapenemases in the isolates that had carbapenem MIC <= 4 mu g/ml and MIC <= 12 mu g/ml were blaOXA-48-type and blaNDM-type respectively. CTX-M-1-like and qnrS were the dominant ESBL and PMQR genes, respectively. This is the first report in which qnrS was described in the isolates from Saudi Arabia. (C) 2017 The Authors. Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz University for Health Sciences. This is an open access article under the CC BY-NC-ND license
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