期刊
JOURNAL OF CELL COMMUNICATION AND SIGNALING
卷 12, 期 4, 页码 661-672出版社
SPRINGER
DOI: 10.1007/s12079-018-0468-0
关键词
Titanium; Polydopamine; BMP-2; PDLSCs; Osteogenic differentiation
类别
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [2015R1A2A2A01006490]
A mussel-inspired polydopamine (PDA), resulting from the oxidative polymerization of dopamine, was reported to be an attractive substrate for advancing biomaterial applications. Thus, this study determined the osteoconductive/osteoinductive properties of titanium (Ti) surfaces coated with PDA and the facilitation of the PDA layer to immobilize bone morphogenetic protein-2 (BMP-2) on Ti substrates. The surface chemistry of PDA or PDA/BMP-2-coated Ti was confirmed by contact angle measurement, scanning electron microscopy (SEM), immunofluorescence staining, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). We verified the osteogenic potential of periodontal ligament stem cells (PDLSCs) cultured on the PDA or PDA/BMP-2-Ti surfaces. The osteogenic differentiation of the PDLSCs was assessed by measuring alkaline phosphatase (ALP) activity, intracellular calcium levels, as well as by evaluating osteocalcin (OCN), osterix (OSX), and runt-related transcription factor 2 (RUNX2) protein levels. The PDLSCs cultured on PDA/BMP-2-Ti showed the highest osteogenic activity compared with those on the control Ti and PDA-coated Ti surfaces. Moreover, PDLSCs on PDA and PDA/BMP-2-Ti expressed increased levels of integrin 1 and actin molecules compared to cells on control Ti. Blocking integrin 1 significantly decreased the osteogenic activity of PDLSCs on PDA/BMP-2 surfaces. This study suggests that the PDA coating can efficiently encourage the immobilization of BMP-2 on Ti surfaces and that this modified Ti substrate highly enhanced the osteogenic differentiation of PDLSCs by integrin-mediated cell-matrix adhesion mechanisms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据