期刊
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
卷 52, 期 11, 页码 8330-8341出版社
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.11-7633
关键词
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资金
- Vistakon, Johnson & Johnson Vision Care, Inc.
- National Institutes of Health [R01EY05681]
- Core Grant [EY02687]
- CTSA [UL1 RR024992]
- Washington University [P30NS057105]
- National Centers of Research Resources [P41-RR00954]
- Research to Prevent Blindness (Departmental of Ophthalmology and Visual Sciences)
PURPOSE. To determine whether class 1 UV-blocking contact lenses protect against UVB radiation-induced damage in a human lens epithelial cell line (HLE B-3) and postmortem human lenses using a proteomics approach. METHODS. HLE B-3 cells were exposed to 6.4 mW/cm(2) UVB radiation at 302 nm for 2 minutes (768 mJ/cm(2)) with or without covering by senofilcon A class 1 UV-blocking contact lenses or lotrafilcon A non-UV-blocking (lotrafilcon A has some UV-blocking ability, albeit minimal) contact lenses. Control cells were not exposed to UVB radiation. Four hours after treatment, cells were analyzed by two-dimensional difference gel electrophoresis and tandem mass spectrometry, and changes in protein abundance were quantified. F-actin and microtubule cytoskeletons were examined by fluorescence staining. In addition, human donor lenses were exposed to UVB radiation at 302 nm for 4 minutes (1536 mJ/cm(2)). Cortical and epithelial cell proteins were scraped from lens surfaces and subjected to the same protein analyses. RESULTS. Senofilcon A lenses were beneficial for protecting HLE B-3 cells against UVB radiation-induced changes in caldesmon 1 isoform, lamin A/C transcript variant 1, DEAD (Asp-Glu-AlaAsp) box polypeptide, beta-actin, glyceraldehyde 3-phosphate dehydrogenase (G3PDH), annexin A2, triose phosphate isomerase, and ubiquitin B precursor. These contact lenses also prevented actin and microtubule cytoskeleton changes typically induced by UVB radiation. Conversely, non-UV-blocking contact lenses were not protective. UVB-irradiated human lenses showed marked reductions in alpha A-crystallin, alpha B-crystallin, aldehyde dehydrogenase 1, beta S-crystallin, beta B2-crystallin, and G3PDH, and UV-absorbing contact lenses significantly prevented these alterations. CONCLUSIONS. Senofilcon A class 1 UV-blocking contact lenses largely prevented UVB-induced changes in protein abundance in lens epithelial cells and in human lenses. (Invest Ophthalmol Vis Sci. 2011;52:8330-8341) DOI: 10.1167/iovs.11-7633
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