期刊
GIGASCIENCE
卷 7, 期 3, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/gigascience/gix135
关键词
Aedes albopictus; C6/36; cell line; genome assembly
资金
- Department of Homeland Security (DHS) [HSHQDC-15-C-B0059]
- National Nature Science Foundation of China [81 420 108 024]
- Natural Science Foundation of Guangdong Province [2014A030312016]
- National Institute of Allergy and Infectious Diseases (NIAIDP) grant [AI123338]
- Intramural Research Program of the National Human Genome Research Institute, National Institutes of Health
- Intramural Research Program of the National Institutes of Health, National Library of Medicine
- DHS Science and Technology Directorate (ST) [HSHQDC-07-C-00020]
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [ZIAHG200398] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI123338] Funding Source: NIH RePORTER
Background: The 50-year-old Aedes albopictus C6/36 cell line is a resource for the detection, amplification, and analysis of mosquito-borne viruses including Zika, dengue, and chikungunya. The cell line is derived from an unknown number of larvae from an unspecified strain of Aedes albopictus mosquitoes. Toward improved utility of the cell line for research in virus transmission, we present an annotated assembly of the C6/36 genome. Results: The C6/36 genome assembly has the largest contig N50 (3.3 Mbp) of any mosquito assembly, presents the sequences of both haplotypes for most of the diploid genome, reveals independent null mutations in both alleles of the Dicer locus, and indicates a male-specific genome. Gene annotation was computed with publicly available mosquito transcript sequences. Gene expression data from cell line RNA sequence identified enrichment of growth-related pathways and conspicuous deficiency in aquaporins and inward rectifier K+ channels. As a test of utility, RNA sequence data from Zika-infected cells were mapped to the C6/36 genome and transcriptome assemblies. Host subtraction reduced the data set by 89%, enabling faster characterization of nonhost reads. Conclusions: The C6/36 genome sequence and annotation should enable additional uses of the cell line to study arbovirus vector interactions and interventions aimed at restricting the spread of human disease.
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