期刊
3 BIOTECH
卷 8, 期 6, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-018-1298-8
关键词
Membrane morphology; Membrane synthesis; Lycopene; Production; Escherichia coli
资金
- National Natural Science Foundation of China [31522002]
- National High Technology Research and Development Program of China [2015AA020202]
- Tianjin Key Technology R&D program of Tianjin Municipal Science and Technology Commission [14ZCZDSY00067]
- Novo Nordisk-Chinese Academy of Sciences (NN-CAS) Research Fund [NN-CAS-2015-2]
The goal of this work was to improve the lycopene storage capacity of the E. coli membrane by engineering both morphological and biosynthetic aspects. First, Almgs, a protein from Acholeplasma laidlawii that is involved in membrane bending is overexpressed to expand the storage space for lycopene, which resulted in a 12% increase of specific lycopene production. Second, several genes related to the membrane-synthesis pathway in E. coli, including plsb, plsc, and dgka, were also overexpressed, which led to a further 13% increase. In addition, membrane separation and component analysis confirmed that the increased amount of lycopene was mainly accumulated within the cell membranes. Finally, by integrating both aforementioned modification strategies, a synergistic effect could be observed which caused a 1.32-fold increase of specific lycopene production, from the 27.5 mg/g of the parent to 36.4 mg/g DCW in the engineered strain. This work demonstrates that membrane engineering is a feasible strategy for increasing the production and accumulation of lycopene in E. coli.
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