期刊
STEM CELL REPORTS
卷 11, 期 1, 页码 288-302出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2018.05.013
关键词
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资金
- Israel Science Foundation
- Israel Academy of Sciences [289/11]
Aiming to unravel the top of the mammary epithelial cell hierarchy, a subset of the CD49f(high) CD24(med) mammary repopulating units (MRUs) was identified by flow cytometry, expressing high levels of CD200 and its receptor CD200R1. These MRUCD200/CD200R1 repopulated a larger area of de-epithelized mammary fat pads than the rest of the MRUs, termed MRUnotCD200/CD200R1. MRUCD200/CD200R1 maintained a much lower number of divergently defined, highly expressed genes and pathways that support better cell growth, development, differentiation, and progenitor activity than their MRUnotCD200/CD200R1 counterparts. A defined profile of hierarchically associated genes supporting a single-lineage hypothesis was confirmed by in vitro mammosphere analysis that assembled 114 genes with decreased expression from MRUCD200/CD200R1 via MRUnotCD200/CD200R1 toward CD200(+)CD200R1(-) and CD200R1(+)CD200(-) cells. About 40% of these genes were shared by a previously published database of upregulated genes in mammary/breast stem cells and may represent the core genes involved in mammary stemness.
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