期刊
STEM CELL REPORTS
卷 10, 期 1, 页码 228-242出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2017.11.016
关键词
-
资金
- US NIH [R01-CA155693]
- Department of Defense [W81XWH-13-1-0352, W81XWH-14-1-0575, W81XWH-16-1-0575]
- CPRIT [RP120380]
- Chinese Ministry of Science and Technology (MOST) [2016YFA0101203]
- RPCI
- NCI [P30CA016056]
- CRPIT [RP120348]
The existence of slow-cycling luminal cells in the prostate has been suggested, but their identity and functional properties remain unknown. Using a bigenic mouse model to earmark, isolate, and characterize the quiescent stem-like cells, we identify a label-retaining cell (LRC) population in the luminal cell layer as luminal progenitors. Molecular and biological characterizations show that these luminal LRCs are significantly enriched in the mouse proximal prostate, exhibit relative dormancy, display bipotency in both in vitro and in vivo assays, and express a stem/progenitor gene signature with resemblance to aggressive prostate cancer. Importantly, these LRCs, compared with bulk luminal cells, maintain a lower level of androgen receptor (AR) expression and are less androgen dependent and also castration resistant in vivo. Finally, analysis of phenotypic markers reveals heterogeneity within the luminal progenitor cell pool. Our study establishes luminal LRCs as progenitors that may serve as a cellular origin for castration-resistant prostate cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据