期刊
PEERJ
卷 6, 期 -, 页码 -出版社
PEERJ INC
DOI: 10.7717/peerj.4931
关键词
Leguminivora glycinivorella; Immune-related gene; RNA interference; Double-stranded RNA; Artificial feeding assay
资金
- China National Novel Transgenic Organisms Breeding Project [2016ZX08004-004-006]
- Chinese National Natural Science Foundation [31201229]
- Foundation for University Key Teacher of the Department of Education of Heilongjiang Province [1253G008]
- Academic Backbone Project of Northeast Agricultural University [15XG03]
- Harbin Science and technology innovation program [2017RAQXJ122]
RNA interference (RNAi) technology may be useful for developing new crop protection strategies against the soybean pod borer (SPB; Leguminivora glycinivorella), which is a critical soybean pest in northeastern Asia. Immune-related genes have been recently identified as potential RNAi targets for controlling insects. However, little is known about these genes or mechanisms underlying their expression in the SPB. In this study, we completed a transcriptome-wide analysis of SPB immune-related genes. We identified 41 genes associated with SPB microbial recognition proteins, immune-related effectors or signalling molecules in immune response pathways (e.g., Toll and immune deficiency pathways). Eleven of these genes were selected for a double-stranded RNA artificial feeding assay. The down-regulated expression levels of LgToll-5-1a and LgPGRP-LB2a resulted in relatively high larval mortality rates and abnormal development. Our data represent a comprehensive genetic resource for immune-related SPB genes, and may contribute to the elucidation of the mechanism regulating innate immunity in Lepidoptera species. Furthermore, two immune-related SPB genes were identified as potential RNAi targets, which may be used in the development of RNA-imediated SPB control methods.
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